goat anti human nectin4 polyclonal antibody (R&D Systems)
Structured Review

Goat Anti Human Nectin4 Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+nectin4+polyclonal+antibody/pm23174504-156-10-15?v=R%26D+Systems
Average 94 stars, based on 32 article reviews
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1) Product Images from "Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor."
Article Title: Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor.
Journal: Virology
doi: 10.1016/j.virol.2012.10.033
Figure Legend Snippet: Fig. 1. Infection of the parental, nectin4-expressing, and SLAM-expressing Vero cells with wild-type CDV strains. (A) Vero/hNectin4 (left panel) and Vero/dNectin4 (right panel) cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) Vero, Vero/ hNectin4, Vero/dNectin4, Vero/hSLAM, and Vero.DogSLAMtag cells were infected with wild-type CDV strains (Ac96I-VDS, 82Con, 55L, M24Cr, and Th12) or mock- infected. At 48 h (Vero, Vero/hNectin4, Vero/dNectin4, and Vero/hSLAM) or 24 h (Vero.DogSLAMtag) post-infection, the cells were stained with the Giemsa solu- tion, and observed under a phase-contrast microscope. (C) Replication kinetics of Ac96I. Vero/hNectin4, Vero/dNectin4, and parental Vero cells were infected with Ac96I at a MOI of 0.01. At various time intervals post-infection, the virus titers were determined by plaque assays.
Techniques Used: Infection, Expressing, Staining, Control, Microscopy, Virus
Figure Legend Snippet: Fig. 2. Amino acid sequence comparison of the V domains of human, dog, and mouse nectin4. Dots indicate identical residues to those of human nectin4.
Techniques Used: Sequencing, Comparison
Figure Legend Snippet: Fig. 4. Replication kinetics in NCI-H358 cells. (A) NCI-H358 cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) NCI-H358 cells were infected with the Ac96I-VDS, 82Con, 55 L, M24Cr, or Th12 CDV strains at a MOI of 0.01. At 5 days post-infection, the virus titers were determined by plaque assays. (C, D) NCI-H358 (C) and II-18 (D) cells were infected with Ac96I-VDS or Ac96I-H358 at a MOI of 0.01. At 1, 3, 5, and 7 days post-infection, the virus titers were determined by plaque assays.
Techniques Used: Staining, Control, Infection, Virus